Two months later.
Chen Chen stood quietly in the laboratory, his face a little pale.
In front of him, a white mouse kept swimming in a Morris water maze.
This little white mouse swims extremely flexibly. Although it looks a little confused, it can always quickly find the right path after making wrong attempts, and finally finds the platform hidden under the water.
No one could tell that these mice had signs of severe neurological damage just two weeks ago.
Watching Experiment Mouse No. 5 arrive on the platform, Chen Chen gently pressed the timer and said expressionlessly, "28 seconds, 2 seconds faster than yesterday."
Afterwards, Chen Chen began to fill in the experimental records:
"Six-month-old genetically modified model mice were treated with surgical transplantation of cloned neural stem cells, and were treated with the myeloid triggering receptor 2 (Trem2) agonist interleukin 4 (IL-4) to induce microglial activation."
"Using immunohistochemistry, Western blotting, and fluorescence quantitative PCR to detect the expression of Trem2 from both protein and gene levels."
"The water maze test shows that model mice show cognitive function recovery in the water maze test."
"In this model, after IL-4 treatment, the expression of Trem2 increased, and the memory and cognitive function impairment of the model mice was greatly improved."
"Results: The activation of Trem2 may promote the reduction of autophagy in microglia through the inflammatory response."
Then Chen Chen bent down and caught the white mouse that was resting on the platform. After drying it, he fixed it on the CT machine and fixed the brain so that it could not rotate.
Then, Chen Chen started the machine, and the CT machine began to scan the brain structure of No. 5.
Comparing the new CT scan with the previous image, we can see that the brain structure of the mouse is much fuller than last week and has undergone huge changes compared to two weeks ago.
If the mouse's brain was like a shriveled walnut two weeks ago, now it has become much fuller, like expanded dough.
Chen Chen casually organized all these data into corresponding categories. He looked at the remaining mice in the experimental cage with the same behavior and covered the experimental cage.
Then, Chen Chen turned around, held the head of rat No. 5, and at the same time pinched the base of the rat's tail with his right hand and pulled gently.
Click!
There was a crisp click sound, and No. 5 in his hand died due to rapid and effective cervical dislocation.
Chen Chen placed the white mouse on the dissecting table and began to dissect its brain.
Ten minutes later, No. 5's brain was completely separated, but it was only then that Chen Chen discovered that the brain of this mouse had not recovered well.
Because a large number of neurons die in Alzheimer's disease, the areas where these nerve cells die produce many connective tissue blocks. These tissue blocks occupy the original space of the brain, making it impossible for the brain to return to its previous state.
"Pity."
Chen Chen shook his head and soaked the brains in a solution filled with formalin.
In the past three months, Chen Chen has used cloning as usual to clone this group of mice, and at the same time, he has used compound treatments in these mouse models that also have Alzheimer's disease.
1. Use the gene overexpression method of TOM1 protein to improve the brain's resistance to inflammation and gradually eliminate inflammation.
2. Use the Solanezumab antibody developed by Eli Lilly to remove Aβ protein and amyloid deposition in the brain.
Although this drug has failed several clinical trials and been proven to be unable to treat Alzheimer's disease, its ability to clear Aβ protein is still worthy of recognition.
3. By injecting neural stem cells extracted from cloned embryos, and using cytokines to activate G2 quiescent stem cells that already exist in the brain, the brain can be stimulated to heal itself.
There is also the last item, which is that in the early stages of Alzheimer's disease, microglia are first activated and actively phagocytose abnormally deposited proteins.
However, if Alzheimer's disease has entered the middle or even late stages, the opposite approach will be adopted, inhibiting the activation of microglia until most of the Aβ deposits are removed using the Solanezumab antibody before microglial activation is carried out.
This is the approach taken by Chen Chen. Through the treatment of inflammation, neural stem cell transplantation, and the intervention of the brain's own immune system, this compound approach can completely suppress Alzheimer's disease in the early and middle stages and make it more effective. It cannot get worse or even get better.
In fact, the actual medical world is not unable to achieve Chen Chen's effect. The only limitation is still cloning. In addition to this limitation, cost is also a factor restricting this treatment.
After all, the cost of this treatment plan alone is sky-high. If applied commercially, a course of treatment would cost millions of dollars.
As for the steric hindrance effect caused by the death of a large number of neurons and the occupation of the brain by connective tissue, Chen Chen was at a loss. Perhaps it would be a good method to remove these connective tissues through craniotomy.
As for NZT-48, due to lack of time and lack of any actual progress, Chen Chen stopped the experiment.
Now that the NZT-48 experiment has stopped, Chen Chen began to call on his assistants to assign some less important tasks. This was how he obtained these experimental results so quickly.
Now that the experiment had produced results, Chen Chen no longer hesitated and directly asked his assistant to bring in the elderly chimpanzees he had prepared earlier.
The scientific community has long discovered that chimpanzees can suffer from the same Alzheimer's disease as humans, so before the experiments on mice were completed, assistants were asked to create chimpanzee Alzheimer's disease models and clone these chimpanzees. .
This can also save a lot of time.
Not only that, Chen Chen also asked one person from the team that protected his parents in Zhongzhou District to go to Shangdu to secretly extract Professor Wang Xi's somatic cells and bring them back to clone Professor Wang Xi. After a few months, Professor Wang Xi really When you arrive at the research center, you can use the cloned neural stem cells immediately.
Putting so much effort into it can be regarded as a way of repaying Professor Wang Xi.
Thinking of this, Chen Chen was just about to conduct the next experiment when suddenly the phone rang.
Chen Chen frowned, picked up his phone and looked at it, only to find that it was a call from the screenwriter of the new movie.
Leaving the laboratory to his assistant, Chen Chen walked out of the laboratory and answered the phone.
"Has it been modified? I understand. I'll check it out later."
It turned out that the screenwriter had revised the script again according to Chen Chen's request and brought it to Chen Chen for review.
This is the task Chen Chen entrusted to the Edwards family.
When he came to his office, without any instructions, Xiao X had already opened his mailbox and projected the script sent by the other party on the wall.
Chen Chen spent a few minutes reading the entire script, and then reluctantly nodded.
The story background of the script is set three years after reality. This was requested by Chen Chen. After all, the USB flash drive only accepts movies that are close to the real-life structure. It is safer to set it directly to the real-life background.
The main line of the story is about a little-known pharmaceutical company in North America. A researcher named Alexander suddenly discovered a small chemical molecule that can completely inhibit Alzheimer's disease by chance.
This small molecule can not only prevent tau nerve tangles and prevent Aβ deposition, but also eliminate inflammation and completely end all symptoms of Alzheimer's disease.
This drug is the core of the story.
The plot is a cliché story in which an unknown dark force got the news from nowhere, and in order to obtain drug samples, he started kidnapping the protagonist's colleagues. After the protagonist found out the situation, he quickly ran away with the drugs and was being hunted all the time.
This script had been passed by Chen Chen several times before. In desperation, the screenwriter removed some parts of the film that were illogical with technology and logic, although these parts were very dramatic and had great selling points.
But this time, it almost met Chen Chen's expectations.
It's a pity that Chen Chen didn't expect that Hollywood was so slow. It took two months from script writing to revision. No matter how fast the remaining month was, it would be impossible to produce the movie.
Fortunately, Chen Chen has found a treatment method to suppress Alzheimer's disease. As long as it can be successful in chimpanzees, Chen Chen can enter clinical trials.
Thinking of this, Chen Chen, as an investor, replied with a "pass" to the other party.
In this way, the Hollywood movie can officially start filming.