In the end, after the physical examination and bone age test of the fifty old men, only thirty-three met the experimental requirements.
The remaining seventeen that did not meet the requirements were naturally returned.
Fortunately, Gorob village could find so many elderly people over fifty years old. I heard that in order to gather enough people, the village chief even went to neighboring villages to negotiate with people for people.
The reason why the elderly are scarce is because of the backward medical level. Although cities and towns are fine, there are really not many people who can live to be over fifty years old in villages.
Namibia official statistics have shown that more than 10% of children in this country are orphans because their parents died of AIDS shortly after giving birth to them.
In Namibia, the national population is only 2.58 million, but the HIV infection rate has reached 21.3%, which means that one-fifth of the people in this country are HIV carriers.
Fortunately, Namibia’s HIV infection rate is not too high in Africa, far lower than Botswana’s 37.3%, Swaziland’s 38.8%, and Lesotho’s 28.9%…
Africa has two world-famous specialties, one is diamonds and the other is AIDS.
Ignoring the dissatisfaction of Gorob Village, Chen Chen directly asked the thirty-three people to take a bath, and then arranged for them to live in the basement. They were provided with good food and drink, but their activity areas were restricted.
At the same time, in the laboratory, Chen Chen recruited a group of experienced assistants and began to clone the cloned human embryos of these old men.
In terms of specific operations, Chen Chen used a more advanced three-dimensional polarized light spindle imaging system. After locating the nucleus of the egg cell, he used a laser to punch holes in the zona pellucida, removing the nucleus in a non-damaging mode to the egg cell, and then Only then can a micromanipulator be used to implant the nucleus of the old man's somatic cell.
It is a pity that the technology of this group of people cannot be compared with Chen Chen. They failed several times during the process, and also failed several times in the process of using electric current and chemical induction. In the end, all the cloning was completed under the guidance of Chen Chen. embryo.
Next, these cloned embryos need 14 days to grow to the blastocyst stage, and then the embryonic stem cells inside are extracted.
The reason why the international community opposes the cloning of human embryos is actually to oppose this step. In the eyes of some people, killing these fertilized eggs is equivalent to killing people, which is ethically unacceptable.
Fortunately, these objections have not hindered the research on cloned embryos. So far, embryonic stem cell cloning technology in various countries is still making great strides forward.
After the embryonic stem cells are extracted, by controlling the growth environment of the embryonic stem cells and manipulating genetically specific gene expression, these embryonic stem cells can be differentiated into the required hematopoietic stem cells and mesenchymal stem cells.
The stem cells cloned through this method are almost identical to the genes of the elderly, will not cause any rejection reaction, and are young and powerful stem cells.
After these elderly people undergo chemotherapy and radiotherapy, they will be implanted into the human body to replace the already aging stem cells in the elderly people's bodies.
Once these young hematopoietic and mesenchymal stem cells return to the bone marrow, they will quickly differentiate into blood cells, muscle cells, liver cells, osteoblasts, adipocytes, chondrocytes, matrix and other human cells to repair and improve the aging damage of the human body. .
This is what stem cells do.
However, for a while, the academic community has been stuck in a huge misunderstanding, which was fermented by the earliest cloned sheep Dolly -
As we all know, Dolly the cloned sheep was the earliest artificially cloned animal in the world. Before Dolly was born, its donor was a Finnish Dorset white-faced sheep.
Experimenters extracted mammary gland cells from the sheep and then removed the cell nuclei to clone Dolly.
In other words, Dolly’s genes are basically the same as those of this Dorset white-faced sheep.
This Finnish Dorset white-faced sheep was 6 years old when its mammary gland cells were extracted.
However, after the birth of Dolly the cloned sheep, it was discovered that Dolly's telomeres were shorter than those of sheep of the same age.
Not only that, the cloned sheep Dolly showed symptoms of premature aging when he was 6 years old. Although people took good care of him, Dolly eventually died.
In the same year, the Dorset white-faced sheep, the donor of Dolly the cloned sheep, also died at the age of twelve.
In the study of lifespan in the biological world, the more mainstream theories are the "telomere theory" and the "free radical theory", both of which have not yet been completely confirmed. However, if telomeres are the key factor affecting lifespan, then the cloning of Dolly the Sheep Death seems to be justified.
Because the nucleus donor of Dolly the cloned sheep was a six-year-old sheep, so although Dolly the cloned sheep was just born, the length of her telomeres has actually been worn down to the point where she only has six years left to live? So why did he die at the same time as the donor 6 years later
This misunderstanding affected the entire academic community at that time, casting a shadow over the entire cloning technology.
Because once this conclusion is true, it means that most of the treatments developed by cloning technology are in vain. No matter how long you clone, the length of telomeres will never change...
This misunderstanding has even affected science fiction works after 2000, causing many science fiction works to invariably set the short life span of clones when setting up clones.
Fortunately, at the end of 2019, this misunderstanding was finally revealed -
In November 2019, Professor Li and his team from the Key Laboratory of Animal Reproductive Regulation and Breeding in Zhongzhou District achieved decisive conclusions in the study of telomeres in cloned animals:
After years of research, Professor Li and his team discovered that telomere reprogramming occurs in cloned embryos during the zygotic genome activation period. Regardless of the telomere length of the donor cell, the reprogramming process can return to the original length, that is, the donor cell. The length itself!
Not only that, Professor Li also discovered that the length of telomeres is actually related to the development of cloned embryos.
During the cloning process, the telomeres of cloned embryos that develop normally are significantly restored, while the telomeres of cloned embryos that develop abnormally are not restored, but become even shorter.
In addition, Professor Li also found that during the culture process of cloned embryos, adding some small molecule substances such as trichostatin A, sodium butyrate, melatonin, etc. can effectively improve the growth of telomeres in a low-oxygen environment. Reprogramming efficiency!
This research result was published in "The FASEB Journal", the journal of the Federation of North American Societies of Experimental Biology, and the paper is titled "Inhibitingrepressiveepigicmodificationpromotestelomererejuvenationinsomatellreprogramming".
At this point, the misunderstanding that the life span of cloned animals is limited by the donor has been completely broken.
As for why Dolly, the cloned sheep, suffered from premature aging and why his telomeres were not long enough? Then the benevolent sees benevolence and the wise see wisdom.
While the cloned embryos of these old men were being cultivated in the laboratory, Dlamini finally sent over the people on Chen Chen's list.
This time Dlamini sent a total of thirty people, all of whom were elites among the elites. An ace like Cheng Chao might not be conspicuous among them.
As long as Chen Chen can subdue this group of wolf-like mercenaries, the foundation for establishing a rapid reaction force in the future will be complete.
Chen Chen's goal is to establish a private armed force capable of global precision strikes in the next few years. Its main purpose is to protect the company's property around the world and destroy any evidence that is detrimental to the company.