After the Ching Ming Festival, Qin Ying came to Benzhou to look for the girl Fang Nan.
Check the missing Fang Nan at the police station, and you can find Fang Nan's address in no time. Qin Ying was very happy in her heart. Her body was recovering day by day, and the wound was not so painful anymore. All these were good news, which made her feel especially hearty.
Qin Ying worked relatively calmly, and guarded near Fang Nan’s home. After seeing Fang Nan’s parents, he recognized the appearance of each other. This is a middle-aged couple in their fifties who both work in the unit. It was a teller from a bank, and the man was a driver from the Sanitation Department.
From the aspect of appearance, there is no similarity to the girl of 27th night. The Fang family and his wife are both medium-looking people. The man is not stalwart and handsome, and the woman is not tall and charming. Even though they are very old, they can still work. He guessed the youthful and beautiful appearance on his face when he was young.
This kind of appearance is really far from the 27-night girl. The girls selected by Old Monster Jiuyou are all very beautiful. It is impossible to choose a medium-looking girl to have sex with him.
Qin Ying couldn't see who the Fang family looked more like, so he had to get their DNA samples. This is not a problem for Qin Ying, who is agile, as long as he passes by the couple.
The result of the DNA comparison surprised Qin Ying, because there was no one with the 27th-night girl who was on the same account.
why is that
Qin Ying's forehead oozes cold sweat, is it the family of the other two girls who died in the underworld? If you want to get DNA samples from those two girls, you have to take a trip to the underworld. There are so many dangers in the underworld, it is not easy to go in and out once.
After thinking about it for a long time, Qin Ying still didn't want to give up this clue, so she found Pang Huihui, the woman of the Fang family. After knocking on the door, Pang Huihui opened the door to Qin Ying. There was only a small gap in the door. Pang Huihui stood in the room and asked: "Who are you looking for?"
"Aunt Pang, I have something to do with you." Qin Ying said with a smile on his face.
"I don't know you, are you selling it?" Pang Huihui said vigilantly.
Qin Ying is speechless. Does she look like a salesman? So he said: "Aunt Pang, I want to talk to you about Fang Nan."
"Fang Nan?" Pang Huihui stunned, and immediately remembered something, and said: "Are you a policeman? Any news about Nan Nan?"
"There is a clue, it is not certain that Fang Nan has been found." Qin Ying could only say vaguely. If Yu Qianqian is not too busy to get out of work, it will be much easier to bring Yu Qianqian around. The identity of the police is still a very good profession in China, and most people give the police face.
Pang Huihui thought for a while, and said, "Then come in." Seeing that Qin Ying is a young girl, her vigilance in her heart relaxed a lot. Even the police officer's ID forgot to check it, so Qin Ying was put into the house.
After sitting down in the living room, Qin Ying asked, "Aunt Pang, do you have Fang Nan's things in your house?"
"what's that?"
"It's just close-fitting underwear or sweaters and trousers."
"Yes, what do you want that thing for?" Pang Huihui asked curiously.
"That's it. Those clothes have dandruff or hair. I take them back and do a DNA test to see if Fang Nan’s information is available. Recently, the state has established a DNA archive. With the archived data, you can find me." Qin Ying looked like a policeman.
"Then you sit down, I'll look for it." Pang Huihui walked into the room.
Qin Ying took a look at this house. It was a house with a history of at least 30 years. The layout was relatively conservative. The decoration was outdated. Many areas showed signs of repair. It was a two-bedroom and one-living design. Pang Huihui entered. Fang Nan should have lived in that house.
Seeing this, Qin Ying couldn't help but shook his head. This place was too unfamiliar to her, without any memory, it was definitely not Qin Ying's home. She saw a photo of a family portrait. The young couple on it was the Fang family couple, and the little girl nestled between the couple was Fang Nan.
Qin Ying looked at Fang Nan in the photo carefully, and felt very familiar, but couldn't remember where she had seen it. She was taken aback. It seemed that Fang Nan must be someone in the middle of twenty-seven nights. Since the appearance has changed since the twenty-seventh night of rebirth, it is different from the past. It is very difficult to recognize who is who from the five senses.
After a while, Pang Huihui walked out with some thick clothes made of underwear and woolen thread. Qin Ying took out a magnifying glass and took a closer look. Because these clothes were cleaned and preserved, there were very few dander and hair on them, and DNA identification was not possible with hair alone. The best were blood drops and flesh. Objects, such things rarely exist on clothes, even if there are blood stains, they will be washed by the owner, and no samples can be collected.
After searching for a full hour, Qin Ying finally found more than a dozen hairs in different positions on her body. She asked Pang Huihui for the comb and toothbrush used by Fang Nan. Unfortunately, these items were cleaned up by Pang Huihui due to too long time. Throw it away at the time. Qin Ying left Pang Huihui a little disappointed and walked out of Fang's house.
Qin Ying still settled down in the suburbs of Benzhou City, cultivated the collected Fang Nan's hair in warm water, then observed it with a microscope, and selected restriction endonucleases paired with probes to cut the long-stranded DNA. , To cut the long DNA chain with a large molecular weight into many small fragments of different lengths.
In a gel electrophoresis instrument with a longer gel plate size, electrophoresis is performed on the DNA fragments after enzymatic hydrolysis, and each digested fragment will be separated in an electric field according to its length. Use alkaline solution to denature the separated double-stranded DNA fragments in the gel plate into single-stranded fragments, and then clamp the gel plate in the nylon membrane to make these single-stranded DNA fragments print, transfer and permanently fix it on the nylon. On the membrane.
Let the radioactive DNA fragments be imprinted with the single-stranded DNA fragments on the nylon membrane for molecular hybridization. When the radioactive film is stacked on the nylon film, the radioactive probe on the nylon film emits X-rays to expose the film, so that the DNA fragments with different lengths hybridized with the probe are developed on the film. Such a characteristic DNA fragment bar graph is the so-called DNA fingerprint.
Because DNA has a non-repetitive personality, at most, the data of ribonucleic acid among close relatives is found to be relatively close, which is an important basis for paternity testing and family genetic inheritance in medicine.
After Qin Ying compared the DNA data, he was surprised to find that it was completely consistent with Qin Ao's data.
In other words, Qin Ao is Fang Nan, but why is Fang Nan’s DNA data not close to his mother Pang Huihui and his father at all? Qin Ying was a little confused, there must be a problem in it.
She was not busy telling Qin Ao the result, but made a call to Leng Xiao's cell phone, explaining her discovery, and said: "Mom, I'm all confused. Why is Pang Huihui's DNA different from Qin Ao? "
"You are really a fool, which means that Qin Ao is not Pang Huihui's biological daughter." Leng Xiao guessed the truth over the phone.
Qin Ying was a little dazed, and said, "Then, Mom, do I need to explain this to Qin Ao?"
"Of course it is necessary. Didn't you say that Fang Nan lived with Pang Huihui for a long time? Maybe Qin Ao was adopted by Pang Huihui since childhood, maybe Qin Ao was an abandoned baby who was adopted by Pang Huihui. You just need to ask Pang Huihui to know all these questions Qin Ao certainly doesn't know her origin. You can continue to help her find her biological parents, Qin Ying, you are doing very well."
It turned out to be like this. Qin Ying struck her head hard. She is really stupid. The society is complicated. There are many family members who are not from the same blood. There are many reasons for this, and there are many families. The members come from several different homes. People may not care about these when they are alive. Once they die, the family member's birth problem will be annihilated with the person's death.
With doubts, Qin Ying found Pang Huihui again, and she said to Pang Huihui: "Fang Nan found it, but I still have a question, that is, Fang Nan is not your biological daughter. What is the reason?"
"What? I really found Nan Nan? Is she okay?" Excited, Pang Huihui tightly grasped Qin Ying's arm, looked at her nervously, and heard the words "not her biological daughter". Pang Huihui then let go of her hands.
He covered his face and shook his head and said: "I don't know, I don't know anything, don't ask me."
Qin Ying's heart sank. It seemed that Qin Ao's birth had many problems, and Pang Huihui's expression was clearly guilt and fear. If it was Qin Ao who was adopted normally, Pang Huihui would definitely not feel afraid.
Seeing Pang Huihui, Qin Ying's mood was very complicated. She still held back her anger and said, "Aunt Pang, don't be afraid. Even if Fang Nan is not your biological daughter, you will eventually raise and grow up. Fang Nan will definitely be able to Thank you."
"No, I don't want her anymore, she is not my daughter, she is not." Pang Huihui shouted to Qin Ying. In order to increase her strength, Pang Huihui also waved her arms to drive Qin Ying away.
If the one in front of him was not Qin Ao's adoptive mother, Qin Ying really wanted to cast a spell on her. Thinking of Qin Ao's violent temper, Qin Ying had dispelled the idea of using spells. Since Pang Huihui denied it all, let Qin Ao solve it by himself, and Qin Ying just cooperated.
Qin Ying sighed deeply and said to Pang Huihui: "Whether you admit it or not, Fang Nan lives in this home, Aunt Pang, let me tell you. Actually, Fang Nan is more reasonable, even if You got Fang Nan by illegal means. She will also miss your nurturing for so many years. It is not easy to raise a child. She is definitely not the kind of person who will avenge her, but you must confess to her. What's the secret, otherwise, even I can't help you." ()