Cai Zixuan looked around and said, "The leader of our team is Wang Ruoxiang."
"Is that the case?" Gu Jun frowned helplessly, thinking it was something, "It's not surprising at all, okay, I'm surprised if it wasn't her."
"That's right, that's Wang Ruoxiang." Cai Zixuan was filled with emotion when he heard the words.
Wang Ruoxiang is the monitor of their class. She is capable, beautiful and has a good personality. Such a goddess of academic dominance, the people who pursue her are of course from the teaching building to the bureau building. Tuhao Jun is one of them. He chased her vigorously back then, but in the end Wang Ruoxiang refused in public: "I'm sorry, we are not the same. Humanoid, I don't like people like you."
Erm, a tall, handsome, rich, and eight-year-old man is actually "this kind of person"
At that time, everyone said that thanks to Tu Haojun and the squad leader, they made them believe in love again.
"The squad leader is still single now." Cai Zixuan obviously didn't know how old a woman he looks now.
"Don't look at me like that." Gu Jun laughed at himself, "It's all over." It's true that it's all over. Tuhao Jun's pursuit of the squad leader is partly because he likes it, and partly because he wants to chase everyone's goddess. The deep stuff doesn't matter.
"Really." Cai Zixuan stopped talking about the monitor and looked at his watch, "It's almost 8 o'clock, I have to go to the laboratory."
"Let's go, I'm going to the laboratory building too." Gu Jun said.
Cai Zixuan immediately showed a knowing look in his eyes, he said just now that he didn't miss the squad leader, now he's going to join in the fun, it's nice to be young.
"What?" Gu Jun was really helpless, he wanted to explain but didn't know where to start, "Forget it..."
"Haojun, you don't need to say it, you understand, once the sea was difficult, I understand."
"You understand a sponge."
The medical laboratory building is located in the northwest of the campus. It is a ten-story white brick building with no distinctive appearance. Even opposite the entrance is a rotten concrete floor that has been in disrepair for a long time. However, there are well-equipped laboratories with various instruments in the building for teachers and students to use for scientific research.
"This way." After entering the building, Cai Zixuan walked in front, introducing the situation to Gu Jun as he walked.
The subject of the experiment conducted by their group is called "Study on Photodynamic Therapy of Tumors with Several Photosensitizers", which is a study of subcutaneously planting tumors in mice, then using different photosensitizers for photodynamic therapy, and then observing the changes in tumors .
The allocated funding this time is only 30,000 yuan, which is too much for an undergraduate research experiment, but an imported pure hotofrin photosensitizer can cost tens of thousands of yuan. In order to make more photosensitizers, Wang Ruoxiang carefully calculated and saved money to set up domestic imitation hotofrin group, doteporfin group, ALA group, plus blank control group, a total of 5 groups of mice.
"The research plan and the opening report are all handled by the team leader." Cai Zixuan couldn't help but sigh, "Wang Ruoxiang really deserves it."
Although Professor Gu is a mentor, he only takes time to give guidance occasionally. For a big guy like him, scoring high-scoring SCI articles is his business. Therefore, the Pioneer Cup still depends on the students themselves. The team leader does the most work, but it is the opportunity that everyone dreams of.
Because if the experiment produces a paper, Wang Ruoxiang is the first author, and Cai Zixuan and the others can only be ranked behind.
"We are still in the first stage, which is to establish an ascites-type tumor-bearing mouse model. It has been cultivated for a week, and it is considered impossible today."
Cai Zixuan talked a lot, and suddenly found that Gu Jun hadn't spoken for a long time, as if he frowned when he heard the word tumor...
"Uh Haojun... You know, the success rate of modeling is not 100%."
Cai Zixuan scratched the bald front of his head, really wondering if Gu Jun understood what he said just now.
"There is nothing in the world that can be 100%." Gu Jun lowered his head and covered his face, friend, I haven't learned enough to be a scumbag, okay
"Indeed." Cai Zixuan was very moved by this sentence, and his face was full of vicissitudes, "Everything that has something to do is like a dream."
Well, Gu Jun doesn't understand now...
During the conversation, the two took the elevator to the eighth floor, where the laboratory used by their group was located.
Walking along the corridor, to the door of the laboratory, Cai Zixuan opened the door and walked in first, only to see that all the team members had arrived, and he was the last one.
"Zixuan, I'll be waiting for you."
"what?"
At this time, everyone saw a person who walked in, and the laboratory suddenly fell silent, only the chirping of mice remained.
Tuhao Jun...
Didn't that mean he dropped out of school to travel the world? Didn't you say he hung up? Not that he...
"Hi everyone." Gu Jun waved his hand to greet everyone with a smile, and looked at Wang Ruoxiang, "Hi, monitor."
Wang Ruoxiang was standing by a laboratory table. She had short black hair and a delicate oval face, although she had no makeup, but she was very pretty. She had a tall figure and a white coat, and she was full of heroism. Seeing him, Wang Ruoxiang frowned slightly, "Hi, Gu Jun." The voice was also nice.
Gu Jun nodded in response to her, saying goodbye to her for three months, the squad leader is still so beautiful, but the old him is no longer.
"Hao Jun was suspended from school for something, and now he is back." Cai Zixuan explained to everyone, and then introduced to Gu Jun: Xu Hai, a clinical major, is an acquaintance, Zhang Haoran, who majored in basic medicine, and He Yuhan, who majored in pharmacy, a group of 5 Everyone is there.
Cai Zixuan asked: "Squad leader, Hao Jun wants to take a look with us, is it okay?"
Wang Ruoxiang pursed her lips silently, and said, "Just stand aside and don't make trouble."
Tuhaojun. She murmured in her heart, remembering that at the beginning of school a few years ago, she had a superficial affection for him... Ugh. This kind of person is delicious, lazy, full of five poisons, rotten wood cannot be carved, and mud cannot support the wall... Mice are not as good! Perhaps only by being dragged into slices for research can it contribute to medicine.
"No." Gu Jun obediently stood aside, but always felt that Wang Ruoxiang's eyes were not kind... an illusion.
A row of mouse cages has been placed on the experimental bench, and each cage contains a group of white Kunming mice. This is the most commonly used experimental animal. It is very docile and small. It weighs only 18-22g at the age of 1-1.5 months, and its body length is less than 10cm. It can be used for experiments.
Except for a cage of model mice, the rest are all blank mice, which Wang Ruoxiang just bought from the experimental animal center of the school. Once modeled successfully, they can come in handy.
Now that the team members have arrived, everyone started working immediately.
crackle! Wang Ruoxiang put on rubber gloves and went to the model mouse cage to catch mice. These mice were numbered by dyeing method, and she caught mouse No. 1. Cai Zixuan and several others were in charge of assisting, recording, videotaping and taking pictures.
"The eighth day of implanting fibrosarcoma in the abdominal cavity of S180 mice." Wang Ruoxiang focused and serious, and lightly pressed the obviously swollen abdomen of No. 1 mouse, and the mouse let out a painful cry. She struggled endlessly, but was held firmly in her hand.
She said: "The ascites of the No. 1 mouse continued to grow, and it was already a frog belly. It had good vitality and did not need to be executed for the time being."
Hearing what she said, the team members were immediately a little excited, and they all leaned over to get a better look, "Is this 'Frog Belly'?" "Amazing."
"As expected of a frog belly, the belly is really big." Cai Zixuan said with emotion.
Although they are all eight-year students, it is actually the first time to do this kind of real scientific research experiment.
"Oh." Gu Jun also leaned over to take a look, and still understood what they were doing.
The reason why ascites-type tumor-bearing mice should be produced first is to obtain a large number of highly active S180 tumor cells. You must know that the original cell line is very expensive, but one tube is only enough to inject one mouse, and its activity is very fragile, and the 4 treatment groups they set up need to cultivate hundreds of mice.
So it is to manufacture first, and then expand. Now the mouse has grown a frog belly and can "retract ascites".
"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him, "Can you walk away? It's in the way."
"Okay." Gu Jun shrugged and walked away, your aura is fine, you can get in the way even one meter away from you.
Cai Zixuan and the others pretended not to see it, and Wang Ruoxiang went on to the next step.
She pierced the abdominal cavity of mouse No. 1 with a 5ml syringe, successfully extracted a tube full of yellow clear ascites, and injected it into an Eppendorf cone-bottomed centrifuge tube, which she then centrifuged with a centrifuge. After centrifugation, you can immediately see a large amount of white sediment at the bottom of the centrifuge tube, which is the tumor cells.
"Mouse No. 1 was successfully modeled." Wang Ruoxiang was relieved and happy, and smiled to the team members: "There are a lot of S180 tumor cells in the ascites!"
"Yeah!" "Haha." They were all young people, no one cared about the mouse's feelings, they cheered, Cai Zixuan praised: "Take the first step to success!"
The first step was successful, and then these cells were given to the mice in the treatment group to create subcutaneous tumors, and then photodynamic therapy was performed after the tumors were formed.
There are still many things to do, they continue to work, remove the supernatant from the centrifuge tube, resuspend and count, adjust the cell concentration...
In order to maintain cell viability, all injections should be completed within one hour. So they were busy with one model mouse and then moved on to the next one.
"Adjust the cell concentration to 1×10^7/ml..."
"Yuhan, you are responsible for taking 0.2 ml of cells per needle."
"Team leader, the anesthetic is prepared!"
Each of them was full of fighting spirit, and no one cared about what Tu Haojun would do anymore, and even forgot that he was standing by and watching.
Gu Jun watched quietly by himself, watching He Yuhan prepare the cell suspension, Cai Zixuan anesthetized the healthy mice with pentobarbital sodium intraperitoneally, Xu Hai and Zhang Haoran marked the mice and removed the hair on the buttocks, and finally Wang Ruoxiang gave Mice were subcutaneously injected with tumor cells in the right buttock…
No. 1, No. 2, No. 3, one after another.
Suddenly, Gu Jun's head cracked and hurt. Could it be a brain stem tumor attack...
The pain intensified suddenly, and he couldn't help screaming: "Ah!" The scene in front of him became blurred, flickering like the shadow of a nightmare.
He seemed to be unable to breathe air, as if he was in a dilapidated laboratory, with centrifuges, microscopes, sterilizers and other instruments lying on the ground, broken test tubes, messy documents, fuzzy photo frames, and rotten animals The corpse... the dust turned all of these into a gray color, without any breath of life, only chaos and dead silence.
The pain in his brain became more and more severe, but the scene became clearer, and he saw something that he couldn't describe clearly.
And those distorted corpses on the ground... Unable to identify what kind of animal it is...
He also saw that there was a line of blood written on the wall inside, and it was the mysterious language on the medicine box, but compared with the standard of printed characters, every stroke of that line of blood had a kind of eerie feeling. weird.
In a daze, Gu Jun suddenly seemed to be able to understand the meaning of the line of blood.
He muttered to himself.