Zhang Lin took out the extraction tube from the liquid nitrogen tank, took out a cryopreservation tube from the lifting tube, and put the lifting tube back carefully, because there were still many cryopreservation tubes of other cells in the lifting tube. After Zhang Lin sealed the lid of the liquid nitrogen tank again, he began a series of patient and meticulous operations.
He first put the cryopreservation tube in a water bath, thawed it quickly with 37°C hot water, then changed the centrifuge tube and centrifuged it in a centrifuge for five minutes, then sat down on the ultra-clean workbench, and put the centrifuge tube mouth into the alcohol lamp. Bake and sterilize, discard the waste liquid, add fresh culture medium with a pipette, and repeatedly blow and mix...
Gu Jun has been watching earnestly from the side, and consulted Senior Brother Zhang if he had any questions, and learned a lot.
Such as the speed control of the centrifuge, the suction and release control of the pipette gun, etc., these resuscitation operations are slightly sloppy, which will affect the survival rate and proliferation speed of the cells.
Finally, Zhang Lin poured the cell suspension into a petri dish, added an appropriate amount of culture medium, and put it in a cell culture incubator at 37°C and 5% CO2.
"Wait, it will take at least two days." Zhang Lin took off his mask and pushed his glasses, "I suggest you keep it for a week, so that the tumor formation rate can be guaranteed."
"Try a batch for those who have been raised for two days." Gu Jun shrugged and said, there is no way, there is not enough time, the system shows that his condition will get worse in 30 days, if it is true, it may be difficult to even get out of bed and walk by then. He said again: "Senior brother, go out for a meal at night? I'll treat you."
"No." Zhang Lin waved his hand, "I'm going to feed the mice with medicine at night, I'm a treat."
…
For the next two days, Gu Jun spent the next two days on campus. Apart from getting together with friends, one was reading books, revising, and preparing for make-up exams. The experimental building takes care of the cells and replaces the culture medium once.
On the morning of the third day, Zhang Lin came to see it in person and said that the cells had grown barely enough, and he helped with subculture and preparation of cell suspension.
And Gu Jun went to the school's experimental animal center. This is actually a large breeding base, located a few hundred meters outside the school campus. It is very lonely, probably because it is filled with the smell of various animals.
He bought five nude mice, more than 100 yuan each, which is quite expensive. Fortunately, he still has 20,000 yuan in savings, but it won't last long.
When the money is used, I hate it. Gu Jun really regrets donating the money so early... The person is still alive, but the money is gone.
cough cough. Each of these nude mice is only the size of two or three fingers together, and they have no hair, and they are packed in a sterile box. Once they come into contact with the outside environment with bacteria, they will be easily infected and die. Therefore, the usual feeding and experimentation process all need to be carried out in a barrier environment.
The barrier environment laboratories of Dongda University are located in the Experimental Animal Research Center. This five-storey building with yellow brick walls stands next to the experimental building.
Gu Jun and Zhang Lin met in the lobby on the first floor of the research center, and booked a small nude mouse room for one hour. This is a small room of only 21 square meters, which is divided into front room and back room. The front room has a laboratory table and instruments, and the back room is a breeding room.
Before entering, they and all the items they brought must be sterilized by ultraviolet light. The two were fully equipped with special full-body protective clothing, masks, gloves, foot covers, etc., and then set foot in the nude mouse room.
Soon, on the laboratory bench in the front room, Gu Jun marked five nude mice by punching holes, and then subcutaneously injected tumor cells into their right buttocks one by one.
Zhang Lin is in charge of assisting. These cells can theoretically form tumors when they hit people. Now seeing Tuhaojun being so enthusiastic about the nude mice, he was sure that the little guy didn't want to play murder.
After all the five nude mice were injected, Gu Jun put them into a breeding cage, and then it was to see if they could form tumors.
"Senior Brother, thank you very much." Gu Jun gave Senior Brother Zhang a thumbs-up. Without the help of Senior Brother, it would not have been so smooth.
"The old Chinese doctor you mentioned..." Zhang Lin still had Conan's vigilance on his face, "Is he really not a liar?"
…
For the next week, Gu Jun continued his previous life rhythm, except that he went to the nude mouse room every day to look at the cage of nude mice. Their feeding is under the unified care of the management staff of the nude mouse room, and the daily board and lodging fee for each mouse is 3 yuan.
He only completed three common tasks this week, because those who needed to be treated could not be done. Although the hospital was right next to him, he was not a doctor yet. The tasks he has completed are all related to medical practice. He already has 5 boxes of pills in his hand, and the proficiency of Tairan's hand has reached the first level (3000/5000).
On the seventh day, Gu Jun walked into the nude mouse room in sterile equipment again, and observed the nude mice one by one.
None of them died. A soybean-sized round tumor had grown under the skin on the right side of the buttock of No. 1. After measurement, the diameter was 0.5 cm; No. 3 and No. 4 also had obvious protrusions, with diameters of 0.3 cm and 0.5cm; No. 2 and No. 5 still have no signs of tumor formation.
So far, he can confirm that out of five nude mice, three have tumors.
Gu Jun set two nude mice with tumors as group A, one nude mouse with tumors as group A, and two nude mice without tumors as group C and group D, and went to the animal center to buy 10 healthy nude mice. Set up group E for 5 animals and group F for 5 animals. Groups A, C and E will all receive the same dose of "Yiwen medicine" (named by him) every day; while groups D, D and F will not receive any medication and will only be used as a control.
That is, nude mice with tumors, nude mice without tumors and healthy nude mice each have a group with medication and a group without medication.
At this time, six cages had been divided into six cages on the experimental platform, and the nude mice were chirping and howling.
Gu Jun was messing around with 25 Yiwen medicine capsules. Since the system stated that one box can prolong life by 5 days, and one box has 5 capsules, it should be one capsule a day. This is the dose for an adult human, and the average weight of these nude mice is less than 20g. If they also take one pill a day, they will probably be sacrificed immediately.
He poured out all the powder in a capsule, weighed the dose, and then converted the dose for nude mice based on his own body weight, made it into a solution, and then diluted it proportionally.
Soon, Gu Jun held the gavage needle filled with the medicine in one hand and a fixed healthy nude mouse in the other hand, and began to gavage the first mouse.
"Hawk, chirp." The nude mouse struggled a little, but he still gave this poor thing a dose of potion, making its contribution to medicine.
At this moment, Gu Jun's heartbeat was a little fast, and he stared at the nude mouse in his hand without blinking.
He hadn't done a toxicity test of the alien medicine until this moment. If it died suddenly, maybe this experiment would be meaningless. And now, he's staring at it for five minutes, then 10 minutes...
Half an hour later, the nude mouse still showed no abnormalities.
"It seems that the foreign medicine has no obvious acute toxicity." Gu Jun let out a sigh of relief, "Chronic toxicity needs to be observed."
Only then did he continue drugging a nude mouse. It took ten minutes to finish feeding the other 7 nude mice in the three drug groups, and he observed them for another hour. All of them had no abnormal reaction, they were still able to move, bark, drink water and eat feed.
"Every rat friend, live on." Gu Jun muttered, pressing his aching head, his condition was obviously getting worse day by day, "Please."
…
On the second and third days, Gu Jun continued to work in the nude mouse room every day.
The tumors of the nude mice in the non-medicated group had grown from 0.3 cm to 0.6 cm, and the body weight and mental state had not changed; while the two nude mice in the drug-treated group A had no tumor size after two days. Change, maintained at 0.5cm.
Gu Jun knew what this might mean, the different culture medicine really had a curative effect! These capsules can significantly inhibit tumor growth.
And the rest of the nude mice that were administered the drug were as usual, which also meant that the foreign medicine seemed to have no adverse reactions and did not cause much damage to body functions.
"Perhaps, there is really salvation this time?" He thought to himself, although the uneasiness still lingered in his heart, a new hope was burning at the same time.
The fourth day, the fifth day... In the blink of an eye, a week has passed.
The tumor of the nude mouse in the group had grown to 1.5cm. The huge tumor was in stark contrast to its thin body. It began to lose weight, and its spirit became bad. However, the condition of the two nude mice in group A continued to be stable, and all the nude mice treated with the drug lived well, no different from the nude mice in the blank control group.
I have to say that the results of this experiment are very satisfying.
The efficacy of Yiwen medicine is better than that of the top tumor small molecule targeting drugs currently on the market.
However, Gu Jun also knew that the adverse reactions of Yiwen medicine could not be completely ruled out, this only proved that it had no side effects in a short period of time. Moreover, this is an animal experiment after all, which is different from human clinical experiments.
However, the result of this experiment was already worth his risk.
This afternoon, after Gu Jun wrote his suicide note, he came to the waiting area of the Emergency Department of the Affiliated Hospital of Dongdae University, and sat down on a rare waiting bench. Surrounded by crowds of patients and their families, most of them were crying children and comforting parents, nurses hurried back and forth, nurses pushed the emergency bed...
There was a lot of noise, and the radio in the emergency department kept calling: "No. 182, Chen Ziyang. No. 183, Wang Yuxuan. No. 184, Huang Zixuan..."
Taking a deep breath, Gu Jun resolutely threw a Yiwen medicine capsule into his mouth, took a sip of mineral water and swallowed it.
"Come on." With a calm face, he looked at the emergency sign on the wall over there, "If there are any toxic reactions, come all of them."